Yokogawa CSU-W1 SoRa Confocal Scanner Unit
Optimize results with the CSU-W1 SoRa Confocal Scanner.
Ideal for super-resolution live cell imaging, the unit allows researchers to observe images in high speed, real time without specific or lengthy sample preparations. In addition, the scanner’s spinning disk technology improves the optical limit by approximately 1.4x and almost doubles the final resolution optical limit.
New option CSU-W1 Uniformizer is released.
- XY resolution exceeding diffraction limit
- Ideal for super-resolution live cell imaging
- Easy to use Technology
- Upgradable from CSU-W1
XY resolution of approx. 120nm*1
XY resolution has been improved by approximately 1.4x the optical limit based on spinning-disk confocal technology.
Furthermore, a final resolution approximately twice the optical limit is realized through deconvolution.
NG108 cell
Image provided by Dr. Kaoru Kato, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
Super-resolution live cell imaging system
Just like the CSU, high-speed real time imaging can be performed with super-resolution.
In addition, live cell imaging is possible, reducing bleaching and phototoxicity.
Movie Play
Real time live cell imaging of mitochondria (10FPS)
Image provided by Dr. Kaoru Kato, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
The CSU is easy to use
Super-resolution images can be observed in real time without any specific preparation of sample.
Deep position observation is made possible through optical sectioning based on confocal technology.
Movie Play
*1 For reference
Details
SoRa super-resolution principle
The image formation in regular confocal microscopes is shown as the product of the illumination PSF (point spread function) and detection PSF. If we consider the image formation on the pinhole at a position D from the optical axis, it is the product of the illumination PSF and detection PSF (as shown), and we can see that information at the position D/2 from the optical axis at the light source is transmitted. That is to say, information at the D/2 position at the light source is magnified to D on the pinhole. In order to correct this, a microlens is fitted and the individual focal points projected onto the pinhole are optically reduced by half, creating an ideal image formation.
By doing so, the resolution is made approximately equal to an ideal confocal microscope, in which the pinhole has been reduced to an infinitesimal size, producing an estimated 1.4x improvement upon regular confocal microscopes.
Configuration when upgrading from the CSU-W1
A SoRa disk can be added to your CSU-W1.
By using a magnification changer for SoRa, it’s possible to perform imaging tailored to your experimental requirements through switching between regular confocal observation and super-resolution observation.
| 1x: | Confocal observation (CSU-W1) |
| 2.8x: | Super-resolution 100x objective lens |
| 4x: | Super-resolution 60x objective lens |
Overview : Confocal scanner unit CSU-W1
Product specification
| Product specification*1 | ||||
|---|---|---|---|---|
| Model | 1 camera model (T1) | 2 camera model (T2) | Split view model (T3) | |
| Loadable model | A SoRa disk can be loaded as disk 2, and disk 1 can be selected (50μｍ or 25μｍ) | |||
| Excitation wavelength | 405nm～640nm | |||
| Observation wavelength | 420nm～680nm | |||
| Effective scan field of view | Depends on the magnification changer for SoRa specification (see below) | |||
| External light / NIR port | An external light port cannot be equipped at the same time as the intermediate magnification switcher The NIR port cannot be used together with a SoRa disk |
|||
| Magnification changer for SoRa specification | |
|---|---|
| Lens-switched light path | 3 light paths switched electronically 1x, 2.8x, 4.0x magnification |
| External dimensions | 425(W)×301.1(L)×122.5(H) mm (excl. protrusions and supporting column) |
| Weight | 13kg |
| Microscope connection | Manufacturer-specific adapter |
| Scan field of view when using magnification changer for SoRa | ||
|---|---|---|
| Magnification changer for SoRa | 2.8ｘ | 4.0ｘ |
| Recommended objective lens | 100ｘ | 60ｘ |
| Effective scan field of view | 61x57μｍ | 71x67μｍ |
| Resolution: : PSF FWHM*2 | |
|---|---|
| XY/Z resolution (optical super-resolution) | 150nm / 320nm |
| XY/Z resolution (after deconvolution) | 120nm / 300nm |
*1 Only items which differ from the CSU-W1 are shown. Specification : Confocal scanner unit CSU-W1
*2 Resolution value is for reference only.
Intelligent Imaging Innovations
(product name: Marianas and VIVO)
Nikon Instruments Inc.
(North and South America)
Nikon Instruments Europe B.V.
(Europe)
Nikon Corporation
(Asia except for Japan)
Resources
Yokogawa held our annual “Yokogawa CSU Spinning Disk Image Competition.” To highlight the research and advancements of students and scientists who participated in MBL,
First annual Yokogawa CSU Spinning Disk Image Competition at MBL 2023
List of Selected Publications for the Wide Field of View Confocal Scanner (CSU-W1)
Downloads
Brochures
Videos
Fast, gentle, and clear - live-cell imaging. Yokogawa's unique scanning method minimizes damage to living cells and organisms and even can capture faint/fast life phenomena.
More than 2,500+ units scanning units sold worldwide. This fast, reliable, and accurate technology has been leading cutting-edge research and supporting researchers around the world for more than two decades.
More information: https://www.yokogawa.com/us/solutions/products-platforms/life-science/spinning-disk-confocal/
#confocal #microlens #microscope #CSU #Yokogawa #livecell
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