Minimally Invasive Intracellular Nano-Injector
This system component automates the penetration and injection*1 of single cells using a nanopipette.
It‘s low invasiveness enables manipulation of live single cells.
The system integrates with multiple manufacturers’ inverted microscopes.*2
Minimally Invasive Single Cell Analysis
- Low Invasiveness Glass pipette with tip size of under 100 nm
- Automated Penetration Automated cell surface detection and penetration (Z direction movement)
- Automated Injection Automated, controller volume injection using electro-osmotic flow
- High Success Rate Approx. 95% success rate of injection*3
- Single-Cell Targeting Enabled injection of selected cells under microscope observation
- Rapid Injection Capable of injecting one cell every 10 seconds*3
*1 Function to aspirate intracellular substances is under development.
*2 Microscope sold separately.
*3 Experiment by Yokogawa.
Details
Accurate Positioning
Long stroke movements and accurate positioning enabled by combining stepping motors and piezo electric actuator.
Automatic Cell Detection and Penetration
Automatic cell detection and penetration with ion current measurement.
Injection Method
Utilizes electro-osmotic flow in the tip of the nanopipette to create a pump effect. The amount of injection is controlled by the duration of the voltage pulse application.
Automated Injection Process
Automatically perform approach, surface detection, injection and retraction of the tip of the nanopipette.
Application Example
- Direct injection of substances such as vector and genome editing tools (CRISPR/Cas9) into the nucleus
- Efficacy/toxicity evaluation of drug candidate molecules
- Other physical injection of reagents and proteins
Fast Injection with High Success Rate
By automating the steps to penetrate the target cell, an injection speed of approximately 10 seconds has been achieved.
Fluorescence was observed in 208 out of 220 (94.6%) HeLa cells where the fluorescent protein was injected (experiment by Yokogawa)
Below: RFP was injected into the HeLa cells, and sequentially observed with fluorescence.
Low-Invasive Injection
The extremely small tip diameter of the nanopipette minimizes damage to the target cell.
Below: RFP was injected into the HeLa cells, and sequentially observed with fluorescence.
Specification
| Item | Specification | |
|---|---|---|
| Basic function | Injection | By electro-osmotic flow at tip of nanopipette |
| Actuator Module | Coarse movement (Motor Actuator) | Stroke:50mm/axis (setting resolution: 0.625μm) |
| Fine movement (Piezo Actuator) | Stroke:100μm/axis (setting resolution: 10nm) | |
| Measurement Module | Voltage generation range | -10V～+10V（setting resolution：10mV） |
| Current measurement range | −900 to +900 nA (setting current range: ±9 V) | |
| External dimensions and weight | Main Controller | 260(W) x 99(H) x 280(D) mm, Approx. 2.8kg |
| Piezoelectric Element Controller | 236(W) x 88(H) x 273(D) mm, approx. 4.6kg | |
| Actuator module | 270*(W) x 219(H) x 245*(D) mm, Approx. 2.2kg * In case the X and Y axes move in the direction of the maximum size |
|
| Measurement module | 85(W) x 30(H) x 43(D) mm, Approx. 0.1kg | |
| Joystick | 100(W) x 145(H) x 144(D) mm, Approx. 0.3kg | |
| Safety Guard | 130(W) x 230(H) 287(D) mm, Approx. 0.7kg | |
| Power consumption | Main Controller ＋ Piezoelectric Controller | Max 100VA |
| Ambient conditions for operation | 15～35℃、20～70%RH without condensation | |
Videos
YOKOGAWA will contribute to technology evolution particularly in measurement and analytical tools to help build a world where researchers will increasingly focus on insightful interpretation of data, and advancing Life Science to benefit humanity.
News
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Press Release Dec 1, 2021 Yokogawa Develops Single Cellome System SS2000 for Subcellular Sampling
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Press Release Mar 18, 2020 Yokogawa Releases SU10 Single Cellome Unit for Use in Biological Research
- For the creation of a smart cell industry
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