Faster, Brighter, and More Versatile
The CSU-X1 is the high speed model of our CSU-series, which are widely recognized as the most powerful tools for live cell imaging.
- World's fastest scanning speed of up to 2,000 fps
- Microlens-enhanced Nipkow disk scanning
- Filter wheel with six filter positions (in high-end model)
- Exchangeable dichroic mirror block and emission filters
- 3 configurations: 1-camera model, 2-camera model, bright field model
- CO2 emissions reduced by 40%
Principles of the Microlens-enhanced Nipkow Disk Scanning Technology
A Nipkow spinning disk containing about 20,000 pinholes and a second spinning disk containing the same number of microlens to focus excitation laser light into each corresponding pinhole are mechanically fixed with a motor, and very rapidly raster scan the field of view with about 1,000 laser beams when rotated. The pinhole and microlens pattern are arranged in our proprietary design to optimize raster scan. Multi-beam scanning with the CSU-X1 not only increases scanning speed, but also results in significantly lower photobleaching and phototoxicity, because multiple excitation needs only a low level of laser power at the specimen to fully excite fluorescence.
Details
Faster
- The world's fastest scanning speed (up to 2,000fps in full-frame).
- Yokogawa's proprietary filter wheel with six filter positions moves to the adjacent position at 33 ms, world's fastest speed.
Brighter
Doubled*1 excitation power efficiency with newly developed beam shaper lens, allows use of lower power lasers, and could reduce camera exposure time.
Triplicated*1 S/N by cutting the background noise by one third, expands capability of really low-light imaging. Significantly brighter*1 images by using most efficient dichroic mirrors and emission filters.
More Versatile -2nd Camera Option*1
You can either simultaneously image two different emission ranges with two cameras, or can selectively use one of the two cameras you installed which is most suitable for your current experimental requirement. For each camera port, you can select to install high-speed filter wheel (Option). In addition to standard C-mount adapter, adapters for 8X8 EMCCD camera and F-mount camera are available (Option).
More Versatile -Bright Field Path Option*2
It allows you to use one camera for both confocal imaging with the CSU-X1 and bright-field (non-confocal) imaging through the bypass light path.
More Versatile—Exchangeable mirror block and exchangeable filters
Easily exchangeable dichroic mirror block and emission filters.
*1 It is necessary to use your choice of commercially available dichroic mirrors for simultaneous multicolor imaging.
*2 The Bright Field Option is not applicable to some microscopy set-up due to steric interference. Please inquire the applicability.
High-end model (6-position filter wheel)
High-end model (12-position filter wheel)
Basic model
| Main unit *1 | Main unit + Brightfield*2 |
Main unit + Secondcamera*2 |
|
|---|---|---|---|
| Confocal scanning method | Microlens-enhanced Nipkow disk scanning | ||
| Scanning speed | Choice:1,500～5,000rpm (Standard) 1,500～10,000rpm (High-speed)*3 |
||
| External synchronization | Scan-speed synchronization through pulse signals Input ： TTL level 300Hz ～ 2KHz Corresponding to Nipkow disk spinnig speed 1,500～10,000rpm*3 |
||
| Excitation wavelangth | 405～647nm | ||
| Second port | - | Brightfield | Second camera |
| Dichroic mirror | Option*4 | ||
| Dichroic mirror switching | Automatic 3CH （Dichroic mirror block can be exchangeable） |
||
| Laser beam input | AFC-connector (Polished 8 degree) | ||
| Optical fiber | Yokogawa's standard Single-mode polarization-preserving fiber*5 | ||
| Filter wheel (Emission side) |
Filter wheel with six filter positions | ||
| Emission filter | Option*4 | ||
| Operation panel | Switch open / close of laser shutter | ||
| External control | RS-232C interface via Control unit | ||
| Microscope mount | C -mount adapter | ||
| Operating environment | 15～40℃ / 20～75% RH | ||
| Power consumption (main unit） |
24VDC 1A max. | ||
| Power consumption (AC adapter） |
Input:100 up to 240VAC±10％, 50 or 60Hz±3Hz, 75Wmax. Output:24VDC 2.5Amax. |
||
| External dimension *6 | 175(W)×328.5(H)×301.5(L)mm | 175(W)×328.5(H)×301.5(L)mm | 175(W)×328.5(H)×301.5(L)mm |
| Weight*7 | 8.9kg | 11.7kg | 13.0kg |
*1 Supplied with a control unit ( for filter wheel ) and a filter wheel.
*2 Supplied with two control units ( one each for filter wheel and for bright field ) and a filter wheel.
*3 Option.
*4 Filters are not included ( Excitation filter , Emission filter ,Dichroic mirror ) . Please inquire as you need.
*5 Supplied with each CSU-X1 main unit.
*6 Excluding protruding parts. Including filter wheel.
| Main unit *1 | Main unit + Brightfield*2 |
Main unit + Secondcamera*2 |
|
|---|---|---|---|
| Confocal scanning method | Microlens-enhanced Nipkow disk scanning | ||
| Scanning speed | Choice:1,500～5,000rpm (Standard) 1,500～10,000rpm (High-speed)*3 |
||
| External synchronization | Scan-speed synchronization through pulse signals Input ： TTL level 300Hz ～ 2KHz Corresponding to Nipkow disk spinnig speed 1,500～10,000rpm*3 |
||
| Excitation wavelangth | 405～647nm | ||
| Second port | - | Brightfield | Second camera |
| Dichroic mirror | Option*4 | ||
| Dichroic mirror switching | Automatic 3CH （Dichroic mirror block can be exchangeable） |
||
| Laser beam input | AFC-connector (Polished 8 degree) | ||
| Optical fiber | Yokogawa's standard Single-mode polarization-preserving fiber*5 | ||
| Filter wheel (Emission side) |
Filter wheel with 12 filter positions | ||
| Emission filter | Option*4 | ||
| Operation panel | Switch open / close of laser shutter | ||
| External control | RS-232C interface via Control unit | ||
| Microscope mount | C -mount adapter | ||
| Operating environment | 15～40℃ / 20～75% RH | ||
| Power consumption (main unit） |
24VDC 1A max. | ||
| Power consumption (AC adapter） |
Input:100 up to 240VAC±10％, 50 or 60Hz±3Hz, 75Wmax. Output:24VDC 2.5Amax. |
||
| External dimension *6 | 258(W)×329.8(H)×213.4(L)mm | 259(W)×374.3(H)×248(L)mm |
309.8(W)×329.8(H)×392(L)mm |
| Weight*7 | 7.8kg | 10.6kg | 12.2kg |
*1 Supplied with a control unit ( for filter wheel ) and a filter wheel.
*2 Supplied with two control units ( one each for filter wheel and for bright field ) and a filter wheel.
*3 Option.
*4 Filters are not included ( Excitation filter , Emission filter ,Dichroic mirror ) . Please inquire as you need.
*5 Supplied with each CSU-X1 main unit.
*6 Excluding protruding parts. Including filter wheel.
| Main unit *1 | Main unit + Brightfield*2 |
Main unit + Secondcamera*2 |
|
|---|---|---|---|
| Confocal scanning method | Microlens-enhanced Nipkow disk scanning | ||
| Scanning speed | Choice:1,500～5,000rpm (Standard) 1,500～5,000rpm (High-speed)*3*4 1,500～10,000rpm (High-speed)*3*4 |
||
| External synchronization | Option*4 | ||
| Excitation wavelangth | 405～647nm | ||
| Second port |
- |
Brightfield | Second camera |
| Dichroic mirror | Option*5 | ||
| Dichroic mirror switching | Manual 1CH （Dichroic mirror block can be exchangeable） |
||
| Laser beam input | AFC-connector (Polished 8 degree) | ||
| Optical fiber | Yokogawa's standard Single-mode polarization-preserving fiber*6 | ||
| Filter wheel (Emission side) |
- | ||
| Emission filter | Option*5 | ||
| Operation panel | Switch open / close of laser shutter | ||
| External control | -*4 | ||
| Microscope mount | C -mount adapter | ||
| Operating environment | 15～40℃ / 20～75% RH | ||
| Power consumption (main unit） |
24VDC 1A max. | ||
| Power consumption (AC adapter） |
Input：100～240 VAC ±10％ ,50 or 60Hz 、75W max. Output ： 24VDC 2.5A max. |
||
| External dimension *6 | 175(W)×328.5(H)×213.4(L) mm | 259(W)×373(H)×213.4(L)mm |
308.5(W)×328.5(H)×213.4(L)mm |
| Weight*7 | 7.5kg | 10.0kg | 10.0kg |
*1 Supplied with a control unit ( for filter wheel ) and a filter wheel.
*2 Supplied with two control units ( one each for filter wheel and for bright field ) and a filter wheel.
*3 Option.
*4 Requires control unit for rotation speed control and external synchronization.
*5 Filters are not included ( Excitation filter , Emission filter ,Dichroic mirror ) . Please inquire as you need.
*6 Supplied with each CSU-X1 main unit.
*7 Excluding protruding parts.
Control unit・Filter Wheel
| Control unit | |||
| Type | for 6-position filter wheel (F1) | for 12-position filter wheel (F2) | for bright field (B1) |
| Operating condition | 15～40℃ 20～75%RH | ||
| Power consumption | Input : 100 up to 240VAC ± 10%, 50 or 60Hz, 200VAmax | ||
| External dimension （ｍｍ） |
213(W)×132(H)×438(L) mm | ||
| Weight( | 5.2 kg | 5.2 kg | 5.1 kg |
| 6-position filter wheel for CSU-X1 | |||
| Operatimg condition | 15～40℃ 20～75%RH | ||
| Power consumption | - | ||
| External dimension | 112(W)×226(D)×100(H) | ||
| Weight | 1.9 kg | ||
| 12-position filter wheel for CSU-X1 | ||||
| Operatimg condition | 15～40℃ 20～75%RH | |||
| Power consumption | - | |||
| External dimension | 154(W)×98(D)×154(H) | |||
| Weight | 2.4 kg | |||
Comparison between target and referenced product
| Target Product： CSU-X1 | Referenced product： CSU22+Filter Wheel | |||||||
|---|---|---|---|---|---|---|---|---|
| Energy (MJ) | CO2Emissions (kg) | NOxEmissions (g) | SOxEmissions (g) | Energy (MJ) | CO2Emissions (kg) | NOxEmissions (g) | SOxEmissions (g) | |
| Raw materials | 2,335.5 | 132.5 | 337.1 | 130.1 | 2,610.0 | 148.0 | 374.9 | 147.1 |
| Components | 10,510.2 | 494.5 | 1,423.4 | 452.8 | 15,329.2 | 728.1 | 2,061.7 | 671.7 |
| Machiningand assembly | 1,542.9 | 68.7 | 214.1 | 56.8 | 2,259.8 | 100.7 | 313.6 | 83.1 |
| Logistics | 2,717.7 | 185.5 | 210.8 | 471.1 | 3,211.7 | 218.9 | 252.3 | 553.9 |
| Consumption | 36,713.5 | 1,635.9 | 5,094.8 | 1,351.5 | 66,087.0 | 2,944.8 | 9,171.0 | 1,126.4 |
| Disposal | -569.9 | -31.9 | -75.7 | -33.6 | -612.8 | -34.5 | -81.2 | -37.4 |
| Total | 53,249.8 | 2,485.3 | 7,204.4 | 2,428.8 | 88,884.8 | 4,106.0 | 12,092.2 | 3,851.5 |
| functional factor | 40.1% | 39.5% | 40.4% | 36.9% | ||||
Note: Value of the referenced product is calculated based on the functional factor. Functional factor：2.16
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Resurse
To investigate interactive dynamics of the intracellular structures and organelles in the stomatal movement through live imaging technique, a CSU system was used to capture 3-dimensional images (XYZN) and time-laps images (XYT) of guard cells.
Faster, Brighter, and More Versatile Confocal Scanner Unit
List of Selected Publications : CSU-X1
Downloads
Broșuri
- Confocal scanner unit CSU-X1 (1.3 MB)
Videoclipuri
YOKOGAWA proprietary Spinning Disk technology enables fast real-time confocal imaging for applications such as high-speed 3D and long-term live cell imaging. These quantifiable imaging analysis are essential tools for modern precision drug discovery.
Over past 20 years, YOKOGAWA proprietary Spinning Disk Confocal technology has been widely used as an indispensable imaging tool among top researchers. The technology enables faster live-cell observation with clearer and less photo-bleaching imaging.
YOKOGAWA will contribute to technology evolution particularly in measurement and analytical tools to help build a world where researchers will increasingly focus on insightful interpretation of data, and advancing Life Science to benefit humanity.
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