Overview
Low glucose cell cultivation (eg. ～1 g/L) for biopharmaceutical production requires frequent sampling and frequent glucose supplementation. The excessive sampling requirement not only reduces the volume of the culture medium, but also is an unnecessary burden on the operators when performed manually. Here, we demonstrated that the Advanced Control Bioreactor System BR1000 can effectively automate mammalian cell cultivation under low glucose conditions.
The BR1000 system controls glucose concentration using model predictive control software architecture. The software algorithms, predicts glucose consumption by leveraging the combined data from direct glucose in-line measurements obtained by near-infrared (NIR) spectroscopy, and from viable cell density data obtained by bio-capacitance measurements.
The BR1000 routinely updates the calibration model using the spectrum data from cultivation to improve the accuracy of glucose monitoring and glucose concentration control. This application note shows that the BR1000 can effectively operate under low glucose cultivation (1 g/L) conditions with CHO cells.