Application: CSU-W1
- ES cell
- Zebrafish
- Brain slice
- Early-stage embryo
ES cell
Ocular cup organ regenerated from mouse ES cells
Data: Mototsugu Eiraku, Ph.D., and Yuiko Hasegawa, Ph.D., Sasai Lab., Organogenesis Neurogenesis group, Center for Developmental Biology, RIKEN
![Ocular cup organ regenerated from mouse ES cells](http://web-material3.yokogawa.com/1/6156/details/42004baa4b9d832280da4dbe6feee328d94ebe84.gif)
3D Play
MIP
YZ plane
Measurement condition | |
---|---|
Z-section/stack | 100um(2um/51slices) |
Fluorescent probe | 640nm:Cy5 |
Pinhole | 25um |
Objective lens | 20x dry |
ES cell colony
Data: Nozomu Takata, Ph.D., Sasai Lab., Organogenesis Neurogenesis group, Center for Developmental Biology, RIKEN
![ES cell colony](http://web-material3.yokogawa.com/1/6156/details/1e049b857c9d42f2c765a7ceeaa9763df0893f4a.gif)
3D Play
MIP
Measurement condition | |
---|---|
Z-section/stack | 50um(1um/51slices) |
Fluorescent probe | GFP(Excitation:488nm) mCherry(Excitation:561nm |
Pinhole | 50um |
Objective lens | 60x oil |
Data: Kazuo Yamagata, Ph.D., Center for Genetic Analysis of Biological Responses, The Research Institute for Microbial Diseases, Osaka University
![ES cell colony](http://web-material3.yokogawa.com/1/6156/details/rev.01_6b61fd0c8e0838846e3c0bbecb4ef6454cc37620.gif)
Timelapse(MIP)
Full-size movie Play
Measurement condition | |
---|---|
Z-sections/ stack |
80um (0.4um/201slices) |
Fluorescent probe | 488nm: H2B-EGFP 561nm: mCherry-MBD-NLS |
Pinhole | 50um |
Objective lens | 60x silicone |
Total time | 490 mins (Interval:10mins) |
Zebrafish
Zebra fish embryo
Data: Makoto Suzuki, Ph.D., and Naoto Ueno, Ph.D., Division of Morphogenesis, National Institute of Basic Biology
![Zebra fish embryo](http://web-material3.yokogawa.com/1/6156/details/c476e5f55bab03ec5ac3addce36d9c3aa9461fff.gif)
3D reconstructed image of the whole embryo
![Zebra fish embryo](http://web-material3.yokogawa.com/1/6156/details/7fe3dfb02207cfd58fd4b03c5bbff42cff54a1f9.gif)
Excerpts from Timelapse(MIP)
All timelapse Play
![Zebra fish embryo](http://web-material3.yokogawa.com/1/6156/details/7058464f2b70c77b283d6d577d79d1d0a8473342.gif)
3D reconstructed embryo
(partial, at high magnification)
![YZ Plane](http://web-material3.yokogawa.com/1/6156/details/a86cbe5bc95885592f5c06f8c3a18b8ef094991e.gif)
YZ Plane
Measurement condition | |
---|---|
Z-Sections/ stack |
Upper left:99um(1um/100slices), Lower left:50um(0.5um/101slices), Right:1slice |
Fluorescent probe | Left: membrane RFP(Excitation:561nm) Right: EB3-GFP(Excitation:488nm) membrane RFP(Excitation:561nm) |
Pinhole | 50um |
Objective lens | Upper left:20x dry,Lower left,right: 60x water |
Zebrafish
Real-Time Visualization of Neuronal Activity
Data: Akira Muto and Koichi Kawakami, Division of Molecular and Developmental Biology, National Institute of Genetics
![zebra fish](http://web-material3.yokogawa.com/1/6156/details/6f1984f13a6befa87e16f0eb7dcc32761c7eeb18.gif)
Excerpts from Timelapse(MIP)
All timelapse movie: Play
![zebra fish](http://web-material3.yokogawa.com/1/6156/details/12483d831d31a8e2ee8717cd290237b1b3d9c206.gif)
Time-line MIP of timelapse images
![zebra fish](http://web-material3.yokogawa.com/1/6156/details/3fb6e988892847bdb6ab62d5c6d6202a9e9b1f99.gif)
3D
![zebra fish](http://web-material3.yokogawa.com/1/6156/details/4cc0c655192076a4163625345f116ea735c68f6b.gif)
Larval zebrafish: The fifth day after fertilization
Pink area: Optic tectum
Measurement condition | |
---|---|
Total time | 152 sec. (Stream: 600 images) |
Excitation | 488nm: GCaMP |
Pinhole | 50um |
Objective lens | 40x water |
Reference: Muto et al., Real-Time Visualization of Neuronal Activity during Perception, Current
Biology 23(4):307-311
Brain slice
Brain slice of mouse fetus
Data: Atsunori Shitamukai, Ph.D., Laboratory for Cell Asymmetry, Center for Developmental Biology, RIKEN
![Brain slice of mouse fetus](http://web-material3.yokogawa.com/1/6156/details/07382a32f653830472a353e612fe3350f095fdac.gif)
3D
Measurement condition | |
---|---|
Z-sections/stack | 15um(0.5um/31slices) |
Fluorescent probe | GFP(Excitation:488nm) RFP(Excitation:561nm) |
Pinhole | 50um |
Objective lens | 60x water LWD |
Total time | 2hours (Interval:1min) |
Early-stage embryo
Early-stage mouse embryo
Data: Kazuo Yamagata, Ph.D., Center for Genetic Analysis of Biological Responses, The Research Institute for Microbial Diseases, Osaka University
![Early stage mouse embryo](http://web-material3.yokogawa.com/1/6156/details/rev.01_3a7539d2c7e6bbcc6ed030a4450c654d0e6c45d9.gif)
Timelapse (MIP) | Full-size movie Play
Measurement condition | |
---|---|
Z-sections/ stack |
100um (1um/101slices) |
Fluorescent probe | 488nm: H2B-EGFP 561nm: mCherry-MBD-NLS |
Pinhole | 50um |
Objective lens | 60x silicone |
Total time | 48 hours (Interval:10mins) |
![Early stage mouse embryo](http://web-material3.yokogawa.com/1/6156/details/0ed77dd3d11912886c026ad6b895c9ddc7373f1d.gif)
Excerpts from Timelapse (MIP of chromosome)
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