Life Science

Visualizing the cell behavioral basis of epithelial morphogenesis and epithelial cancer progression

Faster, Deeper, and Clearer -in vivo molecular imaging technology-

Discovering the Basic Principles of Life through the Live Imaging of C. elegans

Closing in on Neuronal Circuit Dynamics through High-speed, fMCI.

New Era in Manmmalian Genetics Research: To utilize the same embryo after long-time 3D observation!

Getting Closer to “Plant Cell World”with High-speed Live Imaging and Image Information Processing.

Spinning Disk Confocal Microscopy for Quantitative Imaging and Multi-Point Fluorescence Fluctuation Spectroscopy.

On-site manipulation of protein activities: Understanding intricate cell signaling pathways.

Use of the spinning disk confocal at the Harvard Medical School microscopy core.

In recent years, single cell analysis has become increasingly popular due to the new development of advanced and high sensitivity analytical methods.
SS2000 is a revolutionary system that can sample subcellular components and a whole cell by using a glass capillary tip with an inner diameter of a few μm while imaging with a confocal microscope.
This application note provides an example of single cell RNA sequencing scRNA seq) from cells sampled by SS2000, and the data is comparable to conventional methods.

In recent years, single-cell studies have become increasingly popular as analytical techniques have become more sensitive and widespread.
The SS2000 is a revolutionary device that can sample intracellular components and single cells using glass capillary tips with a diameter of several micrometers while performing confocal microscope imaging. These application notes introduce examples of sampling for various cell types.

In recent years, research on single cells has become increasingly popular. With more sensitive analytical techniques, it has become possible to analyze specific intracellular components such as organelles, even at the single-cell level.
The SS2000 is an innovative system that can sample subcellular intracellular components using a glass capillary having a tip diameter of only a few micrometers while imaging with a confocal microscope.
In this application note, we introduce a case where after drug treatment, intracellular components were sampled by the SS2000 and analyzed by single-cell mass spectrometry.

Comparison between CSU and conventional LSM in 4D movies.

To investigate interactive dynamics of the intracellular structures and organelles in the stomatal movement through live imaging technique, a CSU system was used to capture 3-dimensional images (XYZN) and time-laps images (XYT) of guard cells.

Cell stage categorized using FucciTime lapse imaging of Fucci-added Hela cells was conducted over 48 hrs at 1 hr intervals. Gating was performed based on the mean intensities of 488 nm and 561 nm for each cell. They were categorized into four stages, and the cell count for each was calculated.

The CV8000 nuclear translocation analysis software enables the analysis of changes in the localization of signal molecules that transfer between cytoplasm and nuclei, such as proteins. The following is an example of the translocation analysis of NFκB, a transcription factor.

The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.

• Colony Formation
• Scratch Wound
• Cytotoxicity
• Neurite Outgrowth
• Co-culture Analysis
• Cell Tracking

Faster, Brighter, and More Versatile Confocal Scanner Unit

CV1000 clears the hurdle in Live Cell Imaging
All-in-one Live cell imaging solution

Welcome to The New World of High Content Analysis
High-throughput Cytological Discovery System

SU10 is a novel technology that enables the delivery of target substances directly into cells (nucleus or cytoplasm) using a "nano" pipette made of a glass capillary with an outer tip diameter of tens of nanometers.

Cell clusters are directly measured with high-throughput 3D imaging Confocal Quantitative Image Cytometer

Wide and Clear
Confocal Scanner Unit

In recent years, research on single cells has become increasingly popular, but with more sensitive analytical techniques, it has become possible to analyze specific intracellular components such as organelles at the single-cell level.

The SS2000 is an innovative system that can sample intracellular components at the single-cell level using a tip (glass capillary) with a diameter of several micrometers while imaging with a confocal microscope. In this application note, we introduce a case in which intracellular components were sampled by SS2000 and genetic analysis was performed by qPCR.

List of Selected Publications : CSU-W1

List of Selected Publications : CQ1

List of Selected Publications : CSU-X1

List of Selected Publications : CV8000, CV7000, CV6000

This "Tutorial" provides overview of this software, from installation through data analysis.

In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

In this tutorial, spheroid diameter and cell (nuclei) count within the spheroid will be analyzed.

In this tutorial, we will learn how to perform time-lapse analysis of objects with little movement using CellPathfinder, through calcium imaging of iPS cell-derived cardiomyocytes.

In this tutorial, we will identify the cell cycles G1-phase, G2/M-phase, etc. using the intranuclear DNA content.

In this tutorial, image analysis of collapsing stress fibers will be performed, and concentration-dependence curves will be drawn for quantitative evaluation.

In this tutorial, we will observe the change in number and length of neurites due to nerve growth factor (NGF) stimulation in PC12 cells.

In this tutorial, intranuclear and intracytoplasmic NFκB will be measured and their ratios calculated, and a dose-response curve will be created.

In this tutorial, we will learn how to perform cell tracking with CellPathfinder through the analysis of test images.

In this tutorial, using images of zebrafish whose blood vessels are labeled with EGFP, tiling of the images and recognition of blood vessels within an arbitrary region will be explained.

The Yokogawa business vision states that the company endeavors to achieve Net-zero emissions, ensure the Well-being of all, and make a transition to a Circular Economy by 2050. 

YOKOGAWA will contribute to technology evolution particularly in measurement and analytical tools to help build a world where researchers will increasingly focus on insightful interpretation of data, and advancing Life Science to benefit humanity.