CV7000S (MEGSZÜNTETVE)

Easy setup of multiple complex measurement parameters

Cell Counting & Cell Searching

• Automatically increases number of imaging points until number of cells imaged reaches to target value.
• Automatically searches appropriate imaging position in a well to acquire image containing target number of cells.

Auto Analysis Start

Automatically transfer the measurement data to analysis WS after image acquisition.
Post-processing includes "Copy", "Move", "Image Correction" and “Starting Auto-analysis” of measurement data.

Wide-view imaging

Wide-view optical scanner has 4x wider image area than our conventional model and provides single-shot image acquisition of whole-well in a 384 wellplate using 4x lens (4 shots using 10x lens).

Comparison of new and conventional image areas

Example of single-shot images of entire 384 wellplate

Wide variety of imaging/display modes

Various imaging/display modes are available to select the best for your application.

Example of display modes

Slice images

MIP

Example of imaging modes 

Confocal

 
Epi-fluorescence

MIP

Reduction of fluorescent cross-talk

Fluorescent cross-talk is an artifact caused by fluorescent signal that is superimposed on other fluorescent color(s) in simultaneous multi-wavelength fluorescent image acquisition.
This artifact can be reduced by image processing.

Example images of fluroescent cross-talk and resultant image of cross-talk reduction

This software allows you to flexibly customize analytical algorithms depending on your application.

Analytical protocols

Ready-to-use standard protocols are available for universal basic analyses. New appropriate protocols can also be built for your application.

Object recognition

Basic and advanced modes are available according to application. In the basic mode, pre-defined parameters such as signal intensity threshold or size can be used to recognize objects. In the advanced mode, appropriate algorithms for object recognition can also be used.
(Preprocessing/Binarization/BinaryTransform/Labeling/LogicalOperating)

Graph of analytical result

Each object is linked to the graph, showing a more detailed analysis of results is possible.

Object link and grouping

This function links multiple different objects of the same cell in different images.

Parallel high speed processing and multi batch processing

Using this function, multiple images of the same measurement can all be processed automatically in the same analytical protocol (recognition algorithms and/or analysis parameters). Moreover, by setting multiple different batch processes, different analyzes of many images can be processed sequentially.

Tiled image analysis

Using tile function, all images of entire well can be reconstructed and analyzed as single image.

We can offer a system tailored to various customers' needs　with most versatile options

Dispenser unit*¹

• Single head
• Disposable-tip(For wellplates of 96 or 384 wells)
• Numerous dispensing functions
• Capable of dispensing different reagents in different time into one well(Multi dispensing)

Stage incubator*¹

• Temperature and CO2 concentration control, humidifier
• Improved environmental control^*3
• Time-lapse imaging over 3 days^*4

High-speed, high-precision XY stage

• Fastest measurement speed in the class (4 minutes for 384 wellplate)
• High-precision positioning

Objective lens*¹

• Automatically switchable
• Up to six lenses
• Automated water supply unit for water immersion lens

Highly sensitive, wide-view with high-resolution, sCMOS camera

• 4x wider image area^*5
• Capable of simultaneous multi-color imaging
• Clearer image with cooled sCMOS camera

*1 Option
*2 Only for 6 and 24 wellplates
*3 CO₂ concentration in stage is calibrated before shipping
*4 Depends on condition of experiment
*5 Compare with our conventional model

Hardware configuration

Plate loaders / lifter

• One-click operation for sample setting and dispensing
• Capable of connecting with external robot systems
• In combination with external robot systems^*1, you can load the wellplates directly from the incubator for imaging and analysis^*2

Bar-code reader

• Useful for easy handling of wellplate information
• Up to two sets（For assay/source plates）

*1 Option
*2 Please contact dealer about the specification

Optical technology

Confocal

Epi-fluorescence

Phase-contrast*¹*²

Wide-view confocal scanner unit

Microlens-enhanced dual Nipkow disk technology significantly reduces photobleaching and phototoxicity in live cell imaging

Principles of the Microlens-enhanced Nipkow Disk Scanning Technology

A Nipkow spinning disk containing about 20,000 pinholes and a subsidiary spinning disk containing the same number of microlenses to focus excitation laser light into each corresponding pinhole are mechanically fixed on a motor, and very rapidly rotated. As a result, high-speed raster scan of the excitation lights on the specimen can be achieved.The pinhole and microlenses are arranged on each disk in our proprietary design to optimize raster scan.
Multi-beam scanning not only increases scanning speed, but also results in significantly lower photobleaching and phototoxicity, because multi-beam excitation needs only low level of laser power on the specimen to fully excite fluorescence.
CV7000 uses wide-view Nipkow disk model which is capable of scanning 4 times wider imaging area with higher resolution and thus capable of ultrafast and high-resolution screening.

Confocal scanner unit CSU-W1

*¹ Option
*² Only for 6 and 24 wellplates

High-throughput Cytological Discovery System