Single Cellome Unit SU10 enables the delivery of substances, such as genome editing tools, directly to the cytoplasm or nucleus of targeted single cells. It can also perform sampling at the single-cell level.
For use with an inverted optical microscope and a stereo microscope type. The microscope is not included with the SU10.
Advantages of SU10
- Single-cell targeting with direct delivery into the nucleus or cytoplasm.
- Nano-point sampling from the nucleus or cytoplasm.
- Minimal damage to cells.
- Automated, high speed, and high success rate.
Automated nano-point injector for single cell : SU10
Details
Single-cell targeting with direct delivery into the nucleus or cytoplasm
- Select cells for delivery while observing under a microscope and deliver substances into the nucleus or cytoplasm of targeted cells.
- Easy Control of XY-position and automatic control of delivery Z-position in software.
Minimal damage to cells
- The nanopipette is a glass pipette with minimum tip outer diameter of several tens of nanometers, minimizing damage to cells.
- Delivery with high cell viability enables live single-cell analysis.
The tip of the nanopipette under an electron microscope.
Automated, high speed, and high success rate
- The SU10 uses automated cell surface detection, insertion, and delivery to the cell. The process takes approximately 10 seconds with a 90% success rate. (Experiment by Yokogawa)
- Operations previously performed manually by experienced researchers are much more manageable with SU10.
Nano-point Sampling
A very small amount can be sampled from a specific part of the cell.
Collected samples can be used in applications such as genetic analysis.
SU10 Automated nano-point delivery operation procedures
Webinar : Nanopipette Technology - A New Tool for Single-Cell Analysis
Our primary focus is introducing a newly developed single-cell manipulation platform (SU-10) using a nanopipette for single-cell injection into living cells. This newly developed technology positions its nanopipette with nanoscale precision, allowing injection and/or aspiration of minute amounts of material into and from individual cells without comprising cell viability. Furthermore, we will display our strategy to develop this new product for single-cell-omics and how this nanopipette technology can analyze multiple analytes, including DNA, RNA, proteins, and other small molecules in basic research or drug discovery.
Watch our webinar about nanopipette technology for free. Register here
Application example
Application example : Delivery of genome editing tools
Delivery of Cas9RNP and Cas9RNP+donor DNA (100b) respectively to GFP-expressing HeLa cells using SU10 and successful knockout of GFP gene/modification to BFP gene.
Application example : Delivery of primary cells
Significantly improves transfection efficiency in cells that have been difficult to transfect
Application example : Delivery to cultured plant cells and plant tissues
- The SU10 can be used in different ways depending on the sample, such as with an inverted microscope for cultured cells and with a stereo microscope for thick tissues.
- Applicable to cells with rigid cell walls or high turgor pressure.
Verified SU10 performance applications
Operation Principle
Automatic cell detection and penetration is a SICM* based technology.
Delivery of solutions and substances into cells is performed by electro-osmosis and electrophoresis.
Q&A
Q1. How is it different from a microinjection system?
A1. The SU10 lowers the damage to a cell with the nanopipette because its tip size is less than 1/10 of a tip used for microinjection.
Automatic detection of cell surface enables a high success rate of insertion to the intended depth of a cell.
The delivery operation uses an electrical method rather than pneumatic or hydraulic pressure.
Q2. How is it different from traditional transfection methods (e.g. transfection reagents, electroporation)?
A2. The SU10 can deliver materials into the selected cells.
The SU10 enables direct delivery of reagents into the cytoplasm or nucleus.
Q3. How is it different from electroporation?
A3. In addition to the above-mentioned “difference from traditional transfection methods”, due to automated cell surface detection, the suspension of cells is not required during injection.
Q4. What is the max volume of injection into the cell?
A4. It is estimated to be tens of femtoliter (fL) per second (1fL=1x10-15L).
The volume can be changed by software settings.
* The delivery volume may vary depending on the solute and vehicle.
Q5. Is the nanopipette disposable?
A5. Yes, but one nanopipette can deliver to 50 cells or more*.
* Experiment using HeLa cells by Yokogawa.
Specification
| Motor actuator | Number of axes | XYZ direction : three axes |
|---|---|---|
| Stroke | XZ : Max. approximately 50mm, Y : Max. approximately 30mm | |
| Setting resolution | 0.625um | |
| Maximum movement speed | 1mm/sec | |
| Piezo actuator | Number of axes | Z direction : one axis |
| Stroke | 500um | |
| Setting resolution | 10nm | |
| Retraction stage | Number of axes | X direction : one axis |
| Stroke | 100mm | |
| Rotary stage | Range of movement | 360° |
| Joystick Controller |
Number of joysticks | For XY operation : one piece, For Z operation : one piece |
| Number of switches | 2-selection toggle switch : one piece, 3-selection toggle switch : one piece | |
| Power voltage | Power voltage | 100 to 240V AC |
| Power frequency | 50/60Hz | |
| Power consumption | 70 VA | |
| External dimensions and weight | Main unit | 337 to 456(W)mm×230(H)mm×377(D)mm, 7.4kg |
| Main controller | 133(W)mm×309(H)mm×364(D)mm, 5.9kg | |
| Joystick controller | 140(W)mm×114(H)mm×144(D)mm, 1.2kg | |
| nanopipette | Tip outer diameter : Several tens of nanometers or less (reference value) (in case of SU10ACC-NP02) |
|
| Ambient operating conditions | Temperature | Main unit and electrode head : 5 to 40°C Other modules : 15 to 35°C |
| Humidity | Main unit and electrode head : 80% RH or less (without condensation) Other modules : 20 to 70% RH (without condensation) |
|
| Installation environment | Keep the system away from direct sunlight. Do not allow it to come in direct contact with water, oil, organic solvents, etc. Make sure there are no flammable, toxic, or corrosive gases. Also, do not use or store the system in locations where sand, dust, or particles have accumulated, where there are sources of strong electromagnetic noises, where fire is used, that are readily exposed to water, or where strong vibrations occur. | |
| Installation posture | Horizontal installation | |
| Altitude | 2000m or less | |
| Storage environment | Temperature | -10 to 50°C |
| Humidity | 95% RH or less (without condensation) | |
| Installation environment | Keep the system away from direct sunlight. Do not allow it to come in direct contact with water, oil, organic solvents, etc. Make sure there are no flammable, toxic, or corrosive gases. Also, do not use or store the system in locations where sand, dust, or particles have accumulated, where there are sources of strong electromagnetic noises, where fire is used, that are readily exposed to water, or where strong vibrations occur. | |
| Operation Environment | For use with an inverted optical microscope. * Microscope is not included with the SU10. Please contact Yokogawa to possibly install the SU10 on a different inverted microscope. Installation examples; Evident IX83, Nikon Ti2, Zeiss Axio Observer |
|
| Software specifications Installation PC requirements |
OS | Windows10，Windows11 |
| USB | One (1) or more Type A2.0 USB ports | |
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Zdroje
This application note will introduce the features of the SU10 and provide examples demonstrating the delivery of genome editing tools (Cas9 RNP) using the technology.
SU10 is a novel technology that enables the delivery of target substances directly into cells (nucleus or cytoplasm) using a "nano" pipette made of a glass capillary with an outer tip diameter of tens of nanometers.
Videa
YOKOGAWA will contribute to technology evolution particularly in measurement and analytical tools to help build a world where researchers will increasingly focus on insightful interpretation of data, and advancing Life Science to benefit humanity.
Novinky
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Tisková zpráva be 18, 2020 Yokogawa Releases SU10 Single Cellome Unit for Use in Biological Research
- For the creation of a smart cell industry
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Tisková zpráva pro 1, 2021 Yokogawa Develops Single Cellome System SS2000 for Subcellular Sampling
A single-cell analysis solution that revolutionizes efficiency in drug discovery research by automating the collection of specific cells and intracellular components
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