Our microscopy and life sciences solutions are designed to support applications from basic research to drug discovery and pre-clinical trials.
Yokogawa’s high content analysis systems and dual spinning disk confocal technologies are used in regenerative medicine, pharmaceutical research, and precision medicine to deliver rapid, high-resolution live cell imaging.
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Spinning Disk Confocal CSU
Using our proprietary dual spinning disk design, Yokogawa’s confocal scanner units transform optical microscopes by enabling real-time live cell imaging.
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High Content Analysis CellVoyager
Our high-content analysis (HCA) systems utilize powerful software to address a wide range of research applications from basic science to complex compound screening.
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OpreX Informatics Manager
Yokogawa Electric's OpreX Informatics Manager is an information-integrated solution that goes beyond electronic lab notebooks by optimizing human and material resource management in terms of skills and scheduling.
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FlowCam Flow Imaging Microscopy
With the FlowCam you can analyze particles accurately, reliably and quickly using automated imaging technology to advance your research, increase productivity, and ensure quality.
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Single-Cell Analysis Solution Single Cellome™
Equipped with a minimally-invasive nanopipette, our Single Cellome Unit is capable of injecting target substances while maintaining the positional information of individual cells.
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Bioreactor
The replacement of manual processes with automation is a trend in the biopharmaceutical industry. For complete automation of fed-batch mammalian cell culture, the control of glucose, a key nutrient source, is critical. Through in-line sensing and model predictive control software, automated feeding and a stable concentration of glucose in bioreactors can be achieved.
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Solutions for Biological Contamination Management
Yokogawa is a proponent of a robust genetic testing method for food, drink, and drug contamination that can be carried out with overwhelming speed and simplicity.
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Principles of Spinning disk confocal
The most common conventional confocal microscopes use a single laser beam to scan a specimen, while the CSU scans the field of view with approximately 1,000 laser beams, by using microlens-enhanced Nipkow-disk scanning: in short, CSU can scan 1,000 times faster.
By using a disk containing microlens arrays in combination with the Nipkow disk, we have succeeded in dramatically improving the light efficiency and thus successfully made real-time confocal imaging of live cells possible.
The expanded and collimated laser beam illuminates the upper disk containing about 20,000 microlenses (microlens array disk). Each microlens focuses the laser beam onto its corresponding pinhole, thus, effectively increasing laser intensity through pinholes placed in the pinhole array disk (Nipkow disk).
With the microlens, backscattering of laser light at the surface of the pinhole disk can be significantly reduced, thus, dramatically increasing the signal to noise ratio (S/N) of confocal images.
About 1,000 laser beams passing through each of the pinholes fill the aperture of the objective lens, and are then focused on the focal plane. Fluorescence generated from the specimen is captured by the objective lens and focused back onto the pinhole disk, transmitted through the same holes to eliminate out-of-focus signals, deflected by the dichroic mirror located between microlens array disk and the Nipkow disk to split fluorescence signal from reflected laser, passed through emission filter and then focused into the image plane in the eyepiece or camera.
The microlens array disk and the Nipkow disk are physically fixed to each other and are rotated to scan the entire field of view at high speeds, thus, making it possible to view confocal fluorescent images in real-time through the eyepiece of the CSU head.
As compared to conventional single point scanning, multi beam scanning by the CSU requires a significantly low level of light intensity per unit area, which results in significantly reduced photo bleaching and phototoxicity in live cells.
Spinning Disk Confocal
Microlens-enhanced Nipkow Disk Technology
Comparison of scanning method
Point Scanning
1 line scan time＝1[ms]
1000 lines/image
Scan lines＝1000 [lines]
1×1000＝1000 [ms]
Disk Scanning by CSU
Rotation Speed＝10000 [rpm]=41.7[rps]
30°Rotation/image
1÷( 41.7×30/360 )＝ 0.5 [ms]
| January | 8,2021 |
Sales release : Advanced Control Bioreactor System BR1000 was released. |
| August | 20,2020 |
Discovering Potential Covid-19 Therapies using High Content Screening Date: Wednesday August 26th 2020 Abstract: |
| June | 5,2020 |
Sales release : High-throughput Cytological Discovery System CV8000 : 20x water immersion lens option was released. |
| March | 18,2020 |
Sales release : Single-cell Analysis Solution Single Cellome Unit SU10 |
| January | 20,2020 |
Sales release : High Content Analysis Software CellPathfinder update and Deep learning option was released. Link to products High Content Analysis Software CellPathfinder |
| January | 15,2020 |
Society for Laboratory Automation and Screening (SLAS) 2020 January 25-29, 2020 We will exhibit high content analysis system "CellVoyager CQ1". Link to products -Poster- 1207-C: |
| December | 6,2019 |
Sales release : A flat-top beam shaper option CSU-W1 Uniformizer |
| November | 18,2019 |
ASCB/EMBO 2019 December 8-10, 2019 We will exhibit Spinning disk confocal "CSU-W1 SoRa" and high content analysis system "CellVoyager CQ1". Link to products -Tech talks- December 9, 3:00-4:00 pm – Theater 2, Learning Center Uniformizer, a new flat-top beam shaper for CSU-W1 & Introduction to new approach for single cell analysis Presenter: Naoki Ando: Product specialist of CSU, Masahiro Kajita: Project manager of “Single Cellome” Yokogawa continues to make improvements to provide the best products for biology researchers. So far, CSU has fulfilled the researchers' wish to take images fast, wide, clear and in super-resolution. This time, we will introduce the new option “Uniformizer” of our product “CSU-W1”. This unit uniformizes the laser illumination and enables quantitative imaging. This provides a large seamless montage image between each field. Furthermore, Yokogawa also takes new challenge for the next field, which is single cell analysis. As a trial, it had prototyped automated single cell sampling system, and it has possible to investigate the molecular characterization of each single cell. This tech talk will introduce 1) ”Uniformizer” and 2) new approach for single cell analysis, which could perform with confocal microscopy. |
| October | 3,2019 |
SLAS 2019 Advanced 3D Human Models and High-Content Analysis Symposium October 21-12, 2019 We will exhibit high content analysis system "CellVoyager CQ1". Link to informations Find out more about SLAS 2019 Advanced 3D Human Models and High-Content Analysis Symposium |
| April | 8,2019 |
FOM 2019 April 14-17, 2019 We will exhibit Spinning disk confocal "CSU-W1 SoRa". Link to products |
| January | 16,2019 |
SLAS 2019 February 4-6, 2019 We will exhibit high content analysis system "CellVoyager". Link to products *Poster presentation is planned. Details will be posted as soon as it is decided. |
| October | 24,2018 |
ASCB/EMBO 2018 December 9-11, 2018 -Tech talks- December 9, 3:00-4:00 pm – Theater 2, Learning Center Super Resolution Confocal Scanner Unit CSU-W1 Sora Presenter: Takuya Azuma: Chief designer of CSU-W1 Sora, Yokogawa will introduce our brand-new product “CSU-W1 SoRa.” This is a spinning disk based super resolution confocal scanner unit. In this talk, we will introduce features and principles of this product and we will show beautiful image samples taken by “CSU-W1 SoRa”. Features of “CSU-W1 SoRa”: 1) XY resolution of approx. 120nm. XY resolution has been improved by approximately 1.4x the optical limit based on spinning-disk confocal technology. Furthermore, a final resolution approximately twice the optical limit is realized through deconvolution. 2) Ideal for super-resolution live cell imaging. Just like the CSU, high-speed real time imaging can be performed with super-resolution. In addition, live cell imaging is possible, reducing bleaching and phototoxicity. 3) The CSU is easy to use. Super-resolution images can be observed in real time without any specific preparation of sample. Deep position observation is made possible through optical sectioning based on confocal technology. 4) Upgradable from CSU-W1. If you already have CSU-W1, you can add SoRa disk. |
| September | 14,2018 |
Sales release : High Content Analysis Software CellPathfinder |
| July | 27,2018 |
Sales release : High-speed Super resolution Confocal Scanner CSU-W1 SoRa |
| June | 11,2018 |
2018 SLAS Europe |
| March | 01,2018 |
Sales release : High Content Data Management System CellLibrarian |
| December | 29,2017 |
SLAS 2018 February 3-7, 2018 |
| December | 29,2017 | Sales news : The Discontinuation of CellVoyagerTM CV7000S High-throughput Cytological Discovery System |
| September | 05,2017 |
Sales release : CellVoyagerTMCV8000 High-throughput Cytological Discovery System |
| January | 19,2017 |
SLAS High-Content Screening Conference 2017 Find out more about SLAS High-Content Screening Conference 2017 |
| April | 04,2016 |
Poster presentation in 3D Cell Culture 2016, 19-21 April 2016, Konzerthaus Freiburg/Germany Yokogawa Electric Corporation will present data obtained by our confocal image cytometer CQ1 in “3D Cell Culture 2016: How close to ‘in vivo’ can we get? Models, Application & Translation”. The poster will show the results of 3D live cell imaging and analysis of the migration and the network formation of HUVEC cells in a multilayered cell sheet. The results demonstrate that CQ1 is an excellent research tool in the field such as regenerative medicine and drug discovery screening. *Data were provided from BioProcess Systems Engineering Lab., Dept.Biotech., Grad. Sch. Eng., Osaka University. |
| February | 10,2016 | Yokogawa Concludes Distribution Agreement with Optec, LLC for Sale of Confocal Quantitative Image Cytometer CQ1 at the markets of OPTEC activity |
| October | 01,2015 | Sales release : Label-free Morphological Analysis Software CellActivision |
Resources
Visualizing the cell behavioral basis of epithelial morphogenesis and epithelial cancer progression
Faster, Deeper, and Clearer -in vivo molecular imaging technology-
Discovering the Basic Principles of Life through the Live Imaging of C. elegans
Closing in on Neuronal Circuit Dynamics through High-speed, fMCI.
New Era in Manmmalian Genetics Research: To utilize the same embryo after long-time 3D observation!
Getting Closer to “Plant Cell World”with High-speed Live Imaging and Image Information Processing.
Spinning Disk Confocal Microscopy for Quantitative Imaging and Multi-Point Fluorescence Fluctuation Spectroscopy.
On-site manipulation of protein activities: Understanding intricate cell signaling pathways.
Use of the spinning disk confocal at the Harvard Medical School microscopy core.
Comparison between CSU and conventional LSM in 4D movies.
To investigate interactive dynamics of the intracellular structures and organelles in the stomatal movement through live imaging technique, a CSU system was used to capture 3-dimensional images (XYZN) and time-laps images (XYT) of guard cells.
Cell stage categorized using FucciTime lapse imaging of Fucci-added Hela cells was conducted over 48 hrs at 1 hr intervals. Gating was performed based on the mean intensities of 488 nm and 561 nm for each cell. They were categorized into four stages, and the cell count for each was calculated.
The CV8000 nuclear translocation analysis software enables the analysis of changes in the localization of signal molecules that transfer between cytoplasm and nuclei, such as proteins. The following is an example of the translocation analysis of NFκB, a transcription factor.
The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
targetingM-cell cycle phase.
- Colony Formation
- Scratch Wound
- Cytotoxicity
- Neurite Outgrowth
- Co-culture Analysis
- Cell Tracking
Faster, Brighter, and More Versatile Confocal Scanner Unit
CV1000 clears the hurdle in Live Cell Imaging
All-in-one Live cell imaging solution
Welcome to The New World of High Content Analysis
High-throughput Cytological Discovery System
Cell clusters are directly measured with high-throughput 3D imaging Confocal Quantitative Image Cytometer
Wide and Clear
Confocal Scanner Unit
List of Selected Publications : CSU-W1
List of Selected Publications : CQ1
List of Selected Publications : CSU-X1
List of Selected Publications : CV8000, CV7000, CV6000
This "Tutorial" provides overview of this software, from installation through data analysis.
In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.
In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.
In this tutorial, spheroid diameter and cell (nuclei) count within the spheroid will be analyzed.
In this tutorial, we will learn how to perform time-lapse analysis of objects with little movement using CellPathfinder, through calcium imaging of iPS cell-derived cardiomyocytes.
In this tutorial, we will identify the cell cycles G1-phase, G2/M-phase, etc. using the intranuclear DNA content.
In this tutorial, image analysis of collapsing stress fibers will be performed, and concentration-dependence curves will be drawn for quantitative evaluation.
In this tutorial, we will observe the change in number and length of neurites due to nerve growth factor (NGF) stimulation in PC12 cells.
In this tutorial, intranuclear and intracytoplasmic NFκB will be measured and their ratios calculated, and a dose-response curve will be created.
In this tutorial, we will learn how to perform cell tracking with CellPathfinder through the analysis of test images.
In this tutorial, using images of zebrafish whose blood vessels are labeled with EGFP, tiling of the images and recognition of blood vessels within an arbitrary region will be explained.
News
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News Brief Apr 26, 2024 Yokogawa Helps to Revolutionize the Field of Single-Cell Lipidomics
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Press Release Jan 31, 2024 Yokogawa Introduces CellVoyager High-Content Analysis System CQ3000
- For greater efficiency in drug discovery and regenerative medicine R&D, and the swift commercialization of new drugs -
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Press Release Jun 22, 2023 Yokogawa to Release OpreX Informatics Manager, Enabling Integrated Management of Experimental Data and Research Resources in the Cloud
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Press Release Dec 1, 2021 Yokogawa Develops Single Cellome System SS2000 for Subcellular Sampling
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Press Release Jun 5, 2020 CSU-W1 Confocal Scanner Unit Arrives at International Space Station
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