Single-Cell Analysis Solutions Single Cellome™

The SU10 is a novel technology that can deliver target substances into cells (nucleus or cytoplasm) by using a "nano" pipette made of glass capillary with an outer tip diameter of just tens of nanometers.

In recent years, single-cell studies have become increasingly popular as analytical techniques have become more sensitive and widespread.
The SS2000 is a revolutionary device that can sample intracellular components and single cells using glass capillary tips with a diameter of several micrometers while performing confocal microscope imaging. These application notes introduce examples of sampling for various cell types.

The SS2000 is a revolutionary system that allows both confocal imaging and sampling of targeted intracellular components or single cells using a glass tip with an inner diameter of a few micrometers. The SS2000 includes an incubator as well as highcontent imaging functions, such as long time-lapse observation, machine learning, and label-free analysis.
The SU10 is an innovative device that delivers target substances directly into cells or into nuclei with a nanopipette that has a tip outer diameter as small as a few tens nm.
This application note shows a case study of sampling and analysis with the SS2000 from cells delivered intracellularly with the SU10.

In recent years, research on single cells has been actively conducted.However, with more sensitive analytical techniques, it is now possible to analyze specific intracellular components at the single-cell level.

In recent years, single-cell studies have become increasingly popular, leading to the development of instruments capable of spatial omics - the integration of samples' 3D locations and their omics data. The SS2000 is a revolutionary device that can collect intracellular components at the single-cell level and image the samples using a confocal microscope. In this application note we introduce the research performed by Dr. Okada of The University of Tokyo, who established an intra single living cell sequencing (iSC-seq) method which uses SS2000 to sample intracellular components from osteoclasts (a type of multinucleated giant cell) and analyzes them through next-generation sequencing.

In recent years, research on single cells has become increasingly popular, but with more sensitive analytical techniques, it has become possible to analyze specific intracellular components such as organelles at the single-cell level.

The SS2000 is an innovative system that can sample intracellular components at the single-cell level using a tip (glass capillary) with a diameter of several micrometers while imaging with a confocal microscope. In this application note, we introduce a case in which intracellular components were sampled by SS2000 and genetic analysis was performed by qPCR.

The SU10 is able to directly deliver substances of interest into single cells’ nuclei or cytoplasm, using a “nano” pipette with a tip outer diameter of only a few tens of nanometers. Fish epithelial keratocytes have been used in many studies of cell motility because of their constant cell shape during their high speed movement. However, these cells cannot be subcultured , which makes it difficult to introduce reagents and genes using conventional methods, such as electroporation.
In this application note, we will demonstrate the use of the SU10 for introducing plasmids and a low membrane permeability fluorescent reagents into keratocytes and the use of the CSU confocal scanner unit for monitoring the cells.

In recent years, single cell analysis has become increasingly popular due to the new development of advanced and high sensitivity analytical methods.
SS2000 is a revolutionary system that can sample subcellular components and a whole cell by using a glass capillary tip with an inner diameter of a few μm while imaging with a confocal microscope.
This application note provides an example of single cell RNA sequencing scRNA seq) from cells sampled by SS2000, and the data is comparable to conventional methods.

SU10 is a novel technology that enables the delivery of target substances directly into cells (nucleus or cytoplasm) using a "nano" pipette made of a glass capillary with an outer tip diameter of tens of nanometers.

The SU10 is able to directly deliver substances of interest into single cell (nucleus or cytoplasm), using a “nano” pipette with a tip  outer diameter of only a few tens of nanometers. This allows fluorescent labeling of live cells and live cell imaging immediately after delivery of the substance, which is not possible with conventional methods. In this application note, we will demonstrate the use of the SU10 for introducing a fluorescent reagent into a cell with low membrane permeability and monitor the intracellular dynamics with the CSU-W1 confocal scanner unit.

This application note will introduce the features of the SU10 and provide examples demonstrating the delivery of genome editing tools (Cas9 RNP) using the technology.

In recent years, research on single cells has become increasingly popular. With more sensitive analytical techniques, it has become possible to analyze specific intracellular components such as organelles, even at the single-cell level.
The SS2000 is an innovative system that can sample subcellular intracellular components using a glass capillary having a tip diameter of only a few micrometers while imaging with a confocal microscope.
In this application note, we introduce a case where after drug treatment, intracellular components were sampled by the SS2000 and analyzed by single-cell mass spectrometry.

YOKOGAWA will contribute to technology evolution particularly in measurement and analytical tools to help build a world where researchers will increasingly focus on insightful interpretation of data, and advancing Life Science to benefit humanity.